The course presents the main concepts of e-Health systems, the security of e-Health systems, and standards for interoperability of e-Health applications. The first part of the course introduces fundamental topics: e-Health terminology and e-Health application types, e-Health standards, interoperability and security issues. The second part presents the most important standards used for e-Health applications, i.e. ISO/IEEE 11073 and the HL7 family of standards, and introduces a hands-on security analysis of IEEE 11073 and HL7 message protocols, types of attacks on e-Health systems, medical data privacy, sensitive data access control policies and protection methods (e.g. data encryption, biometric authentication, role/attribute policies).